First, the layout (first barrier) The design is based on the space provided by the experimental site, and the microbial experimental area is designed with a construction area of ​​23 m2 as an example. There should be a P2 main laboratory, a one more buffer room, one two more buffer room, and one wash and disinfection room. The design makes full use of the existing space and is laid out according to the relevant national specifications and the special requirements of the experimental site. Considering that biosafety laboratories should simultaneously implement the requirements of primary barrier and secondary barrier, the primary barrier is mainly reflected in the layout and maintenance structure of the microbial experimental zone, and the secondary barrier is mainly reflected in the air conditioning purification system. Separating the flow of people from the logistics in terms of layout, preventing cross-infection between people and objects: the experimental operator enters the P2 main laboratory through a more buffer room and two more buffers; the experimental objects enter and exit through two interlocking transfer windows. In the P2 main laboratory, one interlocking transfer window is located between the main laboratory and the preparation room, and the other is located between the main laboratory and the washing and disinfecting room to ensure that the cleansing objects are free from infection and contaminants are ensured. Pass into the washing and disinfecting chamber as soon as possible for disinfection and sterilization. The entire layout can fully meet the national requirements and standards and the final requirements of the experimental site, and make full use of the construction space divided by the experimental site, equipped with various functions according to the requirements of the laboratory operating procedures, the operation route is convenient and fast, each experiment The planar dimensions of the chamber are assigned according to various experimental functions. Second, the envelope structure, decoration and decoration The main frame of the laboratory is made of color steel plate glass partition, the color is dull white, the thickness of color steel is 0.426mm on each side, the fireproof grade of color steel rock wool sandwich panel is not burning; the fireproof grade of polystyrene color steel sandwich panel is Flame retardant. The thickness of the partition glass is 5mm; the R25mm aluminum alloy arc line is used to prevent the deposition of dust window material; the R50mm aluminum alloy fillet is used on the inside of all two-dimensional joints, and the R100mm aluminum is used on the outside of the exposed two-dimensional connection line. The alloy fillet is connected; the three-dimensional joint of the color steel plate uses a three-dimensional joint transition, and the color steel plate and the wall surface are connected by an aluminum alloy slot aluminum. The ceiling material is also a color steel plate. The indoor floor of the microbial area is made of imported PVC with no gap welding, and has the characteristics of corrosion resistance, strong wear resistance, good flatness and easy cleaning. The ground corner line is decorated with an angled aluminum material, which is beautiful and rigorous. Through the scientific design and careful construction, the whole laboratory will form a sterile surface and inner shell which are firm, seamless, smooth, beautiful, non-reflective, dust-free, rust-free, moisture-proof, anti-bacterial and excellent in performance. Thereby solving problems such as air tightness and cleaning. All materials are processed and formed in the factory according to the design drawings, and shipped to the site for quick installation. All the equipment in the room is embedded in the wall, so that the entire laboratory wall surface is smooth and smooth, preventing dust accumulation and convenient cleaning and disinfection. Third, the basic configuration of the microbiology laboratory In addition to solving the problem of air purification, the purification laboratory should also provide as much convenience as possible for the experimental work. Therefore, some necessary laboratory equipment should be considered in the design. 1. Interlocking transfer window: There are two transfer windows in the P2 laboratory. The safety of laboratory logistics is guaranteed. There are UV lamps in the interlocking transfer window to disinfect the contaminated items before they are taken out of the laboratory. The interlocking transfer window also ensures the isolation of outdoor and indoor air, which facilitates the transfer of articles by the experimenter and reduces the number of times the experimenter enters and exits the laboratory. The transfer window can be controlled indoors and outdoors. 2. Automatic door closer: According to the requirements of national regulations, an automatic door closer is installed on the clean laboratory door. 3. Interlocking door: The main laboratory sets up a set of interlocking electronic interlocks. When one of the doors is not closed, the other door will not open, thus buffering the airflow entering the clean room. The interlocking door is also equipped with an emergency switch. When an accident occurs, pressing the emergency switch facilitates the laboratory personnel to escape from the scene as soon as possible. 4, multi-function microcomputer control instrument: as the laboratory's total control cabinet, including temperature and humidity adjustment and display, start and stop sequence of the exhaust fan, lighting switch, UV lamp control and so on. The temperature adjustment display can accurately display the temperature in the laboratory, and the adjustment is convenient, and the temperature error is small. 5, micro pressure difference table: According to the requirements of biosafety laboratory specifications, P2 laboratory is equipped with a set of micro differential pressure control system, which can display the indoor and outdoor pressure difference, and manually control the pressure difference according to requirements, according to the change of indoor and outdoor pressure difference Adjust the differential pressure to within safe limits. 6, socket: according to laboratory requirements. A sufficient number of single-phase and three-phase outlets should be configured in each laboratory depending on the application. The sockets are all embedded and airtight. The imported brand-name multi-functional dustproof, waterproof and anti-leakage combination power supply board is installed in an advanced process. 7. UV lamp: Considering the disinfection requirements of the clean room, two UV disinfection lamps should be installed in the P2 main laboratory to facilitate the physical disinfection of the experiment personnel before and after the experiment. (1) Human body pyroelectric infrared sensor: In order to facilitate the use of the experimental personnel and save power, an infrared detector is placed at the door of the buffer room to sense the heat of the human body, and the fan is automatically turned on to send out the clean air and automatically closes. (2) Scrubbing station and sewage treatment device: According to the needs of the laboratory, there is a washing table and a sewage treatment device in the washing and disinfecting room. The sink and the sewage hose are made of acid-resistant PP material, and the faucet is a laboratory-dedicated triple. Faucet. (3) Eye wash and induction faucet: According to the requirements of the specification, an eye wash device is arranged on the washing table of the microbial experimental area, and the eye of the experimenter can be used to flush the eyes in case of contact with dangerous substances. In order to facilitate the experimenter, the induction faucet can automatically sense the body heat, release the water and automatically delay the closure. (4) Sample car: In order to facilitate the laboratory personnel to move samples and other experimental supplies, a small sample car should be specially arranged in the scrubbing and disinfecting room, which is a stainless steel structure with two layers above and below, which is small and convenient to push. (5) drip truck: According to the experimental supplies, after drying and disinfection, the drip tank should be arranged in the washing and disinfecting room. Some scrubbing and disinfecting utensils can be placed on the drip truck to drip dry, and the drip tank is equipped with There is a bucket to collect the dripping water. The drip truck is removable and has a stainless steel construction. (6) Experimental side table: The laboratory must be equipped with a test bench for placing experimental equipment and laboratory supplies. The test bench is made of acid and alkali resistant physical and chemical core plates, and the slide is made of high quality materials. Steel-wood structure, with drawers and cabinets under the countertop for easy placement of laboratory supplies, aesthetics and laboratory coordination. (7) Biosafety cabinet (BSC-II-A2): According to the national regulations of the biosafety laboratory, the P2 laboratory should be equipped with a biological safety cabinet. Biosafety cabinets are the primary isolation devices used in biological laboratories to prevent the spread of harmful aerosols. The protection provided based on samples of personnel, environment and test samples varies according to their grade. Class II A2 biosafety cabinets provide comprehensive protection for personnel, test samples, and the environment for high-risk microorganisms, toxic substances, and radioactive oligonucleotides. Class II A2 is a front-opening biosafety cabinet that protects the worker by vertical airflow, protects the sample by multi-layer HEPA filtration, and protects the environment by HEPA filtration. The airflow is settled from the top of the cabinet through the HEPA intake filter. After the gas reaches the working surface, about 50% of the gas is sucked into the grille at the rear of the cabinet. The rest of the gas meets the indoor air at the front grille, and 30% of the air passes. The HEPA exhaust filter is drained, leaving 70% of the air mixed with fresh air by HEPA filtration to form a sterile work area that effectively prevents cross-contamination. The indoor air and the HEPA filtered air create a gas barrier, which isolates the worker and the sample, protects the tester and keeps the sample free from contamination. Fourth, P2 biosafety laboratory technical indicators (secondary barrier) The biological clean room should strictly abide by the People's Republic of China health industry standard WS233---2002 "General guidelines for biosafety in microbiology and biomedical laboratories" and the National National Standard GB19489-2004 "General Requirements for Laboratory Biosafety". The assembly standard is based on the degree of biohazard in the biological factor analysis process of each professional laboratory and the classification of biosafety levels according to national standards. Users are often referred to as P1, P2, P3, P4 or It is called first level, second level, third level and fourth level. 1, grading standards Grading of biosafety laboratories 2. Secondary Biosafety Laboratory The biological clean room is two-stage, divided into two buffer rooms (which can double as a dressing room) and one operation room (with an area of ​​12.4M2) according to the layout. The production process must meet the health industry standard WS233- of the People's Republic of China. ---2002: General Guidelines for Biosafety in Microbiology and Biomedical Laboratories. The main technical parameters are as follows: air outlet speed: ≥0.35m/s, clean level seven; difference between operation room and outdoor atmospheric pressure (static pressure difference) ≥-5Pa; temperature 23----30°C adjustable, total noise ≤ 65db in the biological clean room temperature, static pressure difference, electrical components, human body sensing system, lighting UV, air supply, exhaust air are controlled by the microcomputer control box. Various indicators can be set in advance; the human body infrared sensing system is used in the buffer room. When the worker enters and exits the sterile room, the fan and the purification system work automatically, and the dusting object is forcibly purified. Operations that may cause aerosols or spillage of pathogenic microorganisms should be performed in a biosafety cabinet (Class II Biosafety Cabinet) using personal protective equipment and associated disinfection facilities. 3, local 100 clean room The specifications and technical specifications of the main components in the local clean room are as follows: air supply fan: air volume 3000 cubic meters / hour, power: 0.45KW, full pressure: 380Pa, noise <65dB; buffer room fan: 2000 cubic meters / hour, power: 0.18KW full pressure: 380Pa, noise <52dB; purification special air conditioner: KFR-35, air heater: 3000W (three-phase); air supply high efficiency filter: 630 * 630 * 80mm (no partition), initial resistance ≤ 240Pa , sodium flame efficiency ≥ 99.99%, dust holding capacity > 800g; buffer between high efficiency filter: 484 * 484 * 80mm (no separator), initial resistance ≤ 240Pa, sodium flame efficiency ≥ 99.99%, dust holding capacity > 500g. Five, purification air conditioning system The microbial clean room is set up to purify the air conditioning system in order to establish a micro-environment with suitable temperature, humidity, air freshness and aseptic cleanness to facilitate experimental operation. The traditional air conditioning system can only control the temperature, and the cleanliness cannot be completely controlled, thus causing the breeding and reproduction of germs. Such an air conditioning system is far from meeting the hygienic requirements of a specific clean environment in a microbiology laboratory. The clean air conditioning system is carefully designed to control the temperature, dust, bacteria, harmful gas concentration and airflow distribution in the laboratory, to ensure the fresh air volume required by the indoor personnel and the reasonable airflow direction in the room, and to Maintain the appropriate gradient pressure and directional flow in the entire microbiology laboratory to fully realize the “aseptic environmental protection system†and reduce all potential infection risks during the experiment. Another outstanding feature of the structural design is that the maintenance of most indoor air conditioning equipment is carried out outdoors, reducing the chances of non-experimental personnel entering the laboratory, reducing pollution and increasing the use rate of the laboratory. 1. Purification of air conditioning system Each clean laboratory is a separate air conditioning system, and the buffer room and purification laboratory are separate, separate air conditioners to prevent internal cross-contamination during the test and to prevent dangerous gases from leaking out. 2, air treatment purification process The indoor return air is cut by the intermediate effect filter (the return air inlet adopts the national patented fixed air direction adjustable air volume return air port, which can be easily disassembled and replaced in the laboratory). Return to the return air section of the purified combined air handler through the return air duct, after cooling or heating, and then pass through the primary and intermediate efficiency filter in the unit (the filter is placed in the positive pressure section of the unit) and sent to the distribution by the air supply duct. The air supply end of the high efficiency filter is sent out. After the outdoor fresh air is treated, the fresh air mixing section of the clean combined air conditioning unit is mixed with the indoor return air. It is then sent to the clean area after being subjected to temperature and humidity treatment by a hygienic purification combined air handler. 3. Air supply mode and airflow organization The end of the laboratory is supplied with a special 484 air supply static pressure box. The air supply static pressure box is filled with high-efficiency low-resistance and non-separator high-efficiency filter, and the bottom is equipped with air supply diffusing orifice plate (the diffusing orifice plate is flat and seamless with the laboratory ceiling); the local 100-level laboratory is equipped with The laminar flow hood adopts a vertical laminar airflow organization with an outer dimension of 1240x635, a high-efficiency filter inside, and a counterflow diffusion orifice plate at the bottom. The return air in the clean room ensures the full flow of the indoor airflow at the bottom of the four corners, which brings a good dilution effect. The buffer room adopts a high-efficiency air supply port to send the return air to the turbulent air flow organization form, which is a single-side bottom return air. 1. Differential pressure control: The negative pressure of the P2 laboratory is distributed in a gradient, and the airflow flows from the clean area to the semi-polluted area to the contaminated area. The indoor negative pressure can reach more than minus 10Pa. 2. Exhaust system: In order to ensure the realization of differential pressure, an exhaust system is installed in the preparation and operation room of P2 laboratory. The external exhaust air leads to the roof of the building, and the air volume control valve is installed on the system. The scrubbing and disinfection room is equipped with a partial exhaust system to ensure fresh air in the washing and disinfecting room. Through a series of air control, including the purification of indoor air, a certain amount of fresh air and the control of the exhaust negative pressure, the laboratory provides a safe and clean air environment. 3, purification air conditioning unit selection Purification laboratory and buffer room use purification combined air conditioning unit. The main features of the unit are as follows: (1) The units are composed of double-layer panels to achieve the best insulation and noise reduction effects. The average noise absorption coefficient is R=39dB. The frame is designed to be sealed with compact high-quality carbon steel. The whole machine is easy to clean, corrosion-resistant and rust-free. (2) The surface cooler used in the unit has a hydrophilic anti-corrosion coating on the surface of the aluminum fins, so it is not easy to form water droplets, which improves the heat exchange efficiency, and is also difficult to accumulate water and bacteria. (3) The unit adopts stainless steel water collecting tray, and the drainage of the water collecting tray adopts the pressure drainage instead of the water seal. No matter what kind of working condition the surface cooler is in, the condensate can be smoothly drained to effectively avoid the condensed water infusion. . (4) Selection of filter; coarse filter adopts plate filter with large dust capacity, imported glass fiber filter material, durable and durable, with cleaning type and interchangeable type, the retention efficiency can reach more than 75%. . The medium efficiency filter adopts a low-resistance filter, and the filter material is ultra-fine polypropylene fiber filter material, which does not cause re-contamination, and has a good protection effect on the end high-efficiency filter. The structure of the filter frame is reasonable, and it is firmly welded to the inner wall of the box, and the seal is strictly sealed without leakage, and the loading and unloading operation of the filter is convenient. Sixth, power distribution system The engineering distribution system is designed for the power supply and distribution system of the laboratory's lighting and power systems and microbiology laboratory air conditioning units. Designed with "Design Code for Power Supply and Distribution System", "Design Specification for Low Voltage Distribution", "Design Standard for Civil Building Lighting", "Electrical Design Code for Civil Buildings", "Technical Specifications for Construction of Biosafety Laboratory", "Laboratory Biosafety" The General Requirements, the General Guidelines for Biosafety in Microbiology and Biomedical Laboratories, and the bidding documents provided at the laboratory are the basis for design. 1. Laboratory power distribution: Designed to set up a distribution sub-box for the bio-safety laboratory, and separate power distribution from the main distribution box to ensure reliable and safe use of the laboratory. In addition, the distribution of other auxiliary laboratories is carried out in a separate mode from the microbiological safety laboratory, thereby further ensuring reliable use of the biosafety laboratory; the lighting distribution of the main laboratory is separate from the outlet distribution. (1) Load level, power supply system and power supply: According to the requirements of the design specifications, the system adopts the TN-S system structure mode, and provides a power box and microcomputer control instrument for each purification laboratory. The outdoor unit, air-conditioning fan, external fan, and heating and cooling accessories for purifying the air conditioner are all controlled by the microcomputer controller and the microcomputer power box. (2) Selection of wire and route laying method: The incoming circuit of the power box is made of ZRBV-220V type wire, which is a fire-resistant wire, which is laid in the wire slot and then put on the flame-retardant wire pipe to lead to the electric equipment. (3) Power distribution equipment and installation method: The power supply box can be mounted on the wall or embedded. The position can be installed according to the site conditions to facilitate the operation and maintenance of the power supply; all switches and sockets are embedded. (4) Lighting system: According to the requirements of the specification, the internal lighting of the laboratory adopts the ceiling-type clean and closed lamp, and there is no strong reflection in the laboratory, and the illumination meets the requirements of relevant national regulations. Considering the aesthetics, the corridor is an embedded grille light panel. Each laboratory is equipped with a separate control switch that is installed at the entrance and exit of the convenient door. The corridor switch is an off-site switch that is installed at both ends of the corridor. 2, communication system: can be installed according to the needs of the experimental site with external communication systems, such as telephone, fax, network and so on. Hair Stylers,hot air styler,hair stylers for short hair,hair dryer and straightener set,mini hair straighteners,air hair styler Ningbo Meirou Electric Appliance Co.,Ltd. , https://www.mrhotairbrush.com