Anti-GLI1 antibody manual operation

FROM: ABCAM

Catalog Number: RS-1206R

Quantity size: 0.2ml (dilute with pH 7.4 0.01 M PBS or diluent of antibody)

Background: Gli1 is a member of the Kruppel family of zinc finger proteins. The Gli transcription factors constitute the final effectors of the Hedgehog signalling pathway, which is critical to vertebrate development. Gli1 may play a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract. Ectopic activation of Gli proteins is linked to tumorigenesis. In addition, both the Ras and Transforming Growth Factor Beta pathways also stimulate and / or induce Gli1 and Gli2 activity independently of Hedgehog ligands.

Anti-GLI1 antibody manual operation

Specificity: · anti-GLI1 is a rabbit monoclonal antibody unconjugated

· Specific for GLI1 of human, mouse, rat

· Use for western blot, elisa, immunoprecipitation

and immunohistochemistry

· Affinity chromatography purification, purity:> 95%

· Isotype: IgG

· Mol wt: 91kDa

Application: · Immunohistochemistry: 1: 100-500

· ELISA: 1: 500-1000

· Western blotting 1: 100-500

· Optimal working dilutions must be determined by the end user.

Anti-GLI1 antibody manual operation

Storage: Store at –20 ºC for one year. Avoid repeated freeze / thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.