1. Development history of optical microscope
As early as the first century BC, people have discovered that when they observe small objects through spherical transparent objects, they can be enlarged and imaged. Later, I gradually realized the law of spherical glass surface that can magnify and image objects.
In 1590, Dutch and Italian eyewear manufacturers had created microscope-like magnifying instruments. Around 1610, while Galileo in Italy and Kepler in Germany studied the telescope, they changed the distance between the objective lens and the eyepiece to obtain a reasonable microscope optical path structure. At that time, optical artisans were engaged in the manufacture, promotion and improvement of microscopes. .
In the middle of the 17th century, Hook in the United Kingdom and Levenhoek in the Netherlands made outstanding contributions to the development of microscopes. Around 1665, Hook added coarse and fine focus adjustment mechanisms, lighting systems, and worktables for specimens to the microscope. These components have been continuously improved and become the basic components of modern microscopes.
From 1673 to 1677, Levin Hook made a single-component magnifying glass high-power microscope, nine of which have been preserved to this day. Hook and Levin Hook have made outstanding achievements in the study of the microstructure of animals and plants using their own microscopes.
The emergence of high-quality achromatic immersion objectives in the 19th century greatly improved the ability of microscopes to observe fine structures. In 1827, Amici was the first to use immersion objectives. In the 1870s, the German Abbe laid the classical theoretical foundation for microscope imaging. These have promoted the rapid development of microscope manufacturing and microscopic observation technology, and provided powerful tools for biologists and medical scientists including Koch and Pasteur to discover bacteria and microorganisms in the second half of the 19th century.
At the same time as the structure of the microscope itself, the microscopic observation technology is also constantly innovating: polarized microscopy appeared in 1850; interference microscopy appeared in 1893; and phase contrast microscopy was created by the Dutch physicist Zernike in 1935 For this, he won the Nobel Prize in Physics for this purpose in 1953.
The classical optical microscope is just a combination of optical components and precision mechanical components. It uses the human eye as a receiver to observe the magnified image. Later, a photographic device was added to the microscope, using a photosensitive film as a receiver that can be recorded and stored. Modern and widespread use of optoelectronic components, TV camera tubes and charge couplers, etc. as the receiver of the microscope, equipped with a microcomputer to form a complete image information acquisition and processing system.
At present, the main microscope manufacturers in the world are: Olympus, Zeiss, Leica, Nikon. Domestic manufacturers mainly include: Jiangnan, McAudi, etc.

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