Coating antigen There are three major categories of natural antigens, recombinant antigens and synthetic polypeptide antigens. A synthetic polypeptide antigen is a synthetically synthesized polypeptide fragment of an amino acid sequence of an antigenic determinant. Generally, it contains only one antigenic determinant, which has high purity and high specificity. However, since the molecular weight is too small, it is often difficult to directly adsorb to the solid phase. Indirect bonding to the surface of the solid support by means of adsorption of the conjugate to the solid support. Coating antibody IgG has a strong adsorption force for polystyrene, the binding occurs on the Fc segment, and the antibody binding site is exposed. Extracted from antiserum or culture medium containing monoclonal antibody. It is necessary to remove the hybrid antibody before it can be used in ELISA to ensure the specificity of the test. The concentration of monoclonal antibody in ascites is higher, and the specificity is also stronger. Therefore, it is not required to be subjected to absorption chromatography and directly coated. In some cases, mixed with a variety of monoclonal antibodies can achieve better results. After the coating solution was added, it was placed in a refrigerator at 4-8 ° C and incubated at 37 ° C for 2 hours. The coating concentration can vary greatly depending on the nature of the carrier and the coating, and each batch of material needs to be selected by experiment and concentration of the enzyme conjugate. Typical protein coating concentrations range from 10 ng/ml to 20 ug/ml. Sanitary Pads,Sanitary Napkin,Best Sanitary Pads,Cotton Sanitary Pads ShanDong YongFang Sanitary Products Co.,Ltd , https://www.sdyongfang.com